dylight 649 conjugated tomato lectin Search Results


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Eurobio lycopersicon esculentum tomato lectin conjugated to dylight 649
Functionality and maturation of blood vessels (A) Fluorescence microscopy of TA muscle after DyLight 649-isolectin intravenous injections and tissue clearing of WT and scramble- and mLVRF-treated mice. Scale bars, 100 μm. (B) Fluorescence microscopy after tissue clearing and αSMA staining of EDL muscle of WT and scramble- and mLVRF-treated mice. Scale bars, 500 μm. (C) αSMA (red) and CD31 (green) immunostaining on transverse sections of triceps muscles from WT and scramble-, mLVRF-, ASO-, and mLVRF+ASO-treated mice. Scale bar, 100 μm. (D) Quantification of the number of αSMA+/CD31+ blood vessels per mm 2 from the αSMA and CD31 cross-section stainings (∗ p < 0.05, ∗∗ p < 0.01; analyzed by one-way ANOVA). Results are expressed as the mean ± SEM ( n = 4–5 per group).
Lycopersicon Esculentum Tomato Lectin Conjugated To Dylight 649, supplied by Eurobio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Functionality and maturation of blood vessels (A) Fluorescence microscopy of TA muscle after DyLight 649-isolectin intravenous injections and tissue clearing of WT and scramble- and mLVRF-treated mice. Scale bars, 100 μm. (B) Fluorescence microscopy after tissue clearing and αSMA staining of EDL muscle of WT and scramble- and mLVRF-treated mice. Scale bars, 500 μm. (C) αSMA (red) and CD31 (green) immunostaining on transverse sections of triceps muscles from WT and scramble-, mLVRF-, ASO-, and mLVRF+ASO-treated mice. Scale bar, 100 μm. (D) Quantification of the number of αSMA+/CD31+ blood vessels per mm 2 from the αSMA and CD31 cross-section stainings (∗ p < 0.05, ∗∗ p < 0.01; analyzed by one-way ANOVA). Results are expressed as the mean ± SEM ( n = 4–5 per group).

Journal: Molecular Therapy. Nucleic Acids

Article Title: Improving angiogenesis ameliorates the efficacy of ASO-based exon skipping for the treatment of Duchenne muscular dystrophy

doi: 10.1016/j.omtn.2026.102834

Figure Lengend Snippet: Functionality and maturation of blood vessels (A) Fluorescence microscopy of TA muscle after DyLight 649-isolectin intravenous injections and tissue clearing of WT and scramble- and mLVRF-treated mice. Scale bars, 100 μm. (B) Fluorescence microscopy after tissue clearing and αSMA staining of EDL muscle of WT and scramble- and mLVRF-treated mice. Scale bars, 500 μm. (C) αSMA (red) and CD31 (green) immunostaining on transverse sections of triceps muscles from WT and scramble-, mLVRF-, ASO-, and mLVRF+ASO-treated mice. Scale bar, 100 μm. (D) Quantification of the number of αSMA+/CD31+ blood vessels per mm 2 from the αSMA and CD31 cross-section stainings (∗ p < 0.05, ∗∗ p < 0.01; analyzed by one-way ANOVA). Results are expressed as the mean ± SEM ( n = 4–5 per group).

Article Snippet: To visualize the vasculature, mice were intravenously injected with 200 μL of Lycopersicon esculentum (tomato) lectin conjugated to DyLight 649 (Eurobio, Les Ulis, France, DL-1178) 30 min prior to sacrifice.

Techniques: Fluorescence, Microscopy, Staining, Immunostaining, Muscles