Journal: PLOS ONE

Article Title: Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images

doi: 10.1371/journal.pone.0289109

Figure Lengend Snippet: Summary of different approaches to visualizing 3D microvasculature in optically cleared tissues.

Article Snippet: FDISCO [ ] , brain, kidney , AlexaFluor647, CD31, lectin-DyLight-649 , Imaris, ImageJ , general metrics (size change, imaging depth, fluorescence quantification).

Techniques: Whisker Assay, Magnetic Cell Separation, Imaging, Fluorescence, Software, Staining, Muscles, Functional Assay

Journal: Biomaterials

Article Title: Protease-Degradable Microgels for Protein Delivery for Vascularization

doi: 10.1016/j.biomaterials.2016.10.044

Figure Lengend Snippet: Microgels functionalized with a fluorescent RGD molecule were injected into the subcutaneous space on the back of C57BL/6 mice. (a) Images of microgel fluorescence at day 0 and day 14. (b) Percent of signal remaining over time compared to day 0. Data was fit with a simply decay model. (c) Representative fluorescent images of skin explants perfused with lectin to label vasculature. Fluorescent images of microgels in skin explants (bottom left). Dotted white line indicates the area represented in the large image. (d) Number of lectin labeled vessels per field of view (n = 4 mice per group). Significance was determined using one-way ANOVA with Tukey post-test, ***p<0.005.

Article Snippet: At 14 day, following injection, functional vasculature was labeled by perfusing anesthetized mice with 1.0 mg/mL Dylight649-labeled tomato lectin (Vector Labs) via tail vein injection.

Techniques: Injection, Fluorescence, Labeling